NOA-GAPmer™ is very efficient in transcript knockdown and exhibit nice dose-dependency.
Knockdown levels of GLDC gene upon transfection with two single siRNAs and TechNOA designed NOA-NMD
and NOA-GAPmer AONs. The transfection was performed on A549 cells with lipofectamine RNAiMAX and
RNA was harvested at 24h post-transfection. The qPCR data for GLDC was normalized against the
housekeeper HPRT and the relative non-targeting controls (non-targeting siRNA, non-targeting NOA-NMD
NOA-GAPmer) set at a value of 1. The results shown are average and standard error of
mean of three biological replicates. While NOA-NMD and NOA-GAPmer fully knockdown the GLDC transcript,
increasing concentrations of siRNA was not able to fully knockdown the transcript.
NOA-NMD™ are steric hindrance antisense oligonucleotides that repress the target transcript
abundance by nonsense-mediated decay (NMD). They are designed to disrupt the transcript reading frame,
resulting in multiple premature termination codons in a manner that induces the NMD machinery. The
interaction between complexes at the exon-exon junction (UPF2 and UPF3) and at the translation termination
(UPF1) mediates target transcript degradation by the exoribonuclease enzyme..
Every base and backbone of our product is modified chemically to enhance its intracellular stability and
binding specificity. This is possible as NOA-NMD™ mechanism of action is non-catalytic – they do not
interact directly with any intracellular components in the NMD process. Consequently, NOA-NMD™ shows
superior specificity, reproducibility and dose-response than siRNA and gapmer, which has limited capacity
for chemical modifications. Doing so however, will abrogate the respective requisite interactions between
siRNA and Dicer/RISC and between gapmer and RNase-H to effect transcript degradation.
NOA-Switch™ are steric hindrance antisense oligonucleotides for inducing a specific
splice-event. Our products are designed for inducing exon skipping, exon inclusion, usage of alternate 3’
and 5’ splice sites beta, usage of mutually exclusive exons beta, intron retention and
restoration beta. When compared to genetic-manipulation based approaches, our products with their
relatively simple protocol will save you significant time and effort.
You may like to use our SpliceMod™ to visualize and compare
the splicing events among various transcripts for the gene of your interest. We have make it easy for you to
order the NOA-Switch™ products that are relevant to your study seamlessly through our viewer. Or you
can contact us directly to order.
More efficient : enhanced effect of intended splice modulation
Very effective : high rate of success
Low concentration : works at low nM concentration and in a nice dose-dependent manner
Very specific : Avoid overexpression of specific isoforms
The commoditization of next-generation sequencing platforms allows routine characterization of transcripts
abundance, and alternate/aberrant splicing events in the transcriptome. It is however, been very difficult
to follow up with functional studies to identify specific splice-events/transcripts that regulate the
cellular phenotype under study, be it normal cell physiology or disease pathology, in a practicable way.
Our functional study panels make it feasible to interrogate multiple splice-event/transcript candidates
simultaneously and efficiently. They can be assembled accordingly to your RNA-Seq results, or to specific
pathways and gene-families of your choice; depending on your candidates, the panels will consist of NOA-Switch™, NOA-NMD™
or a combination of both product groups. The oligonucleotides can be plated easily on a multi-well
cell-culture plate for multiplex functional studies.